Coordinate Transcription and V(D)J Recombination of the Kappa Immunoglobulin Light-Chain Locus: NF-κB-Dependent and -Independent Pathways of Activation

• DOI: 10.1128/mcb.17.7.3477
• Publication Date: 2015-10-09T22:08:17Z
• Authors: Darin P. O’Brien; Eugene M. Oltz; Brian G. Van Ness
• Keywords: Transcription; Sp3 transcription factor; General transcription factor

To further elucidate the potential role of mitogens and cytokines in regulation kappa immunoglobulin light-chain locus, we have characterized activation transcription factor binding, germ line transcription, DNase I hypersensitivity, Vκ-to-Jκ recombination upon induction model pre-B-cell lines. We find that both lipopolysaccharide (LPS) gamma interferon (IFN-γ) are capable activating locus. also transforming growth β is completely inhibiting LPS but has no apparent effect on including NF-κB. address functional NF-κB IFN-γ these events, blocked nuclear translocation by overexpression a dominant negative mutant IκB-α (IκB ∆N). Overexpression IκB∆N protein results an inhibition not recombination. Our demonstrate necessary sufficient for at kappa. These suggest required or important establishing there multiple independent pathways