A Conserved p38 Mitogen-Activated Protein Kinase Pathway Regulates Drosophila Immunity Gene Expression

• DOI: 10.1128/mcb.18.6.3527
• Publication Date: 2015-10-10T00:32:00Z
• Authors: Zhiqiang Stanley Han; Hervé Enslen; Xiaodi Hu; Xiangjun Meng; I-Huan Wu; Tamera Barrett; Roger J. Davis; Y. Tony Ip
• Keywords: MAPK14; MAP2K7; ASK1; MAPK7; c-Raf; Cyclin-dependent kinase 9; MAPKAPK2; Schneider 2 cells

Accumulating evidence suggests that the insect and mammalian innate immune response is mediated by homologous regulatory components.Proinflammatory cytokines bacterial lipopolysaccharide stimulate immunity activating transcription factors such as NF-B AP-1.One of responses evoked these stimuli initiation a kinase cascade leads to phosphorylation p38 mitogen-activated protein (MAP) on Thr Tyr within motif Thr-Gly-Tyr, which located subdomain VIII.We have investigated possible involvement MAP pathway in Drosophila response.Two genes are highly were molecularly cloned characterized.Furthermore, encode two novel kinases, D-MKK3 D-MKK4, identified.D-MKK3 an efficient activator both while D-MKK4 D-JNK but not D-p38.These data establish indeed possesses conserved signaling pathway.We examined role D-p38 kinases regulation immunity.The results revealed one functions attenuate antimicrobial peptide gene expression following exposure lipopolysaccharide.