Cleavage of Eukaryotic Translation Initiation Factor 4G by Exogenously Added Hybrid Proteins Containing Poliovirus 2Apro in HeLa Cells: Effects on Gene Expression

• DOI: 10.1128/mcb.19.4.2445
• Publication Date: 2015-10-26T10:18:19Z
• Authors: Isabel Novoa; Luis Carrasco
• Keywords: Cleavage (geology); HeLa; Eukaryotic translation

Efficient cleavage of both forms eukaryotic initiation factor 4G (eIF4G-1 and eIF4G-2) has been achieved in HeLa cells by incubation with hybrid proteins containing poliovirus 2Apro. Entry these into is promoted adenovirus particles. Substantial levels ongoing translation on preexisting cellular mRNAs still continue for several hours after eIF4G degradation. Treatment control hypertonic medium causes an inhibition that reversed upon restoration to normal medium. Protein synthesis not restored lacking intact treatment. Notably, induction heat shock occurs pretreated 2Apro, suggesting transcription takes place even the presence cleaved eIF4G. Finally, luciferase was examined a cell line bearing gene under tetracycline-regulated promoter. Transcription transport mRNA cytoplasm at eIF4G-deficient cells. However, strongly inhibited These findings indicate necessary engaged exception but are being translated.