Transcriptional Activation by NF-κB Requires Multiple Coactivators

• DOI: 10.1128/mcb.19.9.6367
• Publication Date: 2015-10-26T10:20:01Z
• Authors: Kelly-Ann Sheppard; David W. Rose; Zaffar K. Haque; Riki Kurokawa; Eileen McInerney; Stefan Westin; Dimitris Thanos; Michael G. Rosenfeld; Christopher K. Glass; Tucker Collins
• Keywords: Transcriptional Activation; 0301 basic medicine; Binding Sites; Saccharomyces cerevisiae Proteins; NF-kappa B; Receptors, Cytoplasmic and Nuclear; Cell Cycle Proteins; 03 medical and health sciences; Nuclear Receptor Coactivator 1; Acetyltransferases; COS Cells; Animals; p300-CBP Transcription Factors; Amino Acid Sequence; Cyclic AMP Response Element-Binding Protein; Histone Acetyltransferases; Transcription Factors

Nuclear factor-kappaB (NF-kappaB) plays a role in the transcriptional regulation of genes involved inflammation and cell survival. In this report we demonstrate that NF-kappaB recruits coactivator complex has striking similarities to recruited by nuclear receptors. Inactivation either cyclic AMP response element binding protein (CREB)-binding (CBP), members p160 family coactivators, or CBP-associated factor (p/CAF) antibody microinjection prevents NF-kappaB-dependent transactivation. Like receptor-dependent gene expression, expression requires specific LXXLL motifs one members, enhancement activity histone acetyltransferase (HAT) p/CAF but not CBP. This is differentially Rel family. The p50 homodimer fails recruit although p50-p65 heterodimeric form transcription assembles integrator complex. These findings provide new mechanistic insights into how dimeric factors differential effect on expression.