Telomerase-Associated Protein TEP1 Is Not Essential for Telomerase Activity or Telomere Length Maintenance In Vivo

• DOI: 10.1128/mcb.20.21.8178-8184.2000
• Publication Date: 2002-07-27T10:03:53Z
• Authors: Yie Liu; Bryan E. Snow; M. Prakash Hande; Gabriela Baerlocher; Valerie A. Kickho...; David Yeung; Andrew Wakeham; Annick Itie; David P. Siderovski; Peter M. Lansdorp; Murray O. Robinson; Lea Harrington
• Keywords: Telomerase RNA component

TEP1 is a mammalian telomerase-associated protein with similarity to the Tetrahymena telomerase p80. Like p80, associated activity and reverse transcriptase, it specifically interacts RNA. To determine role of mTep1 in function vivo, we generated mouse embryonic stem (ES) cells mice lacking mTep1. ThemTep1-deficient (mTep1−/−) were viable bred for seven successive generations no obvious phenotypic abnormalities. All murine tissues from mTep1−/− possessed level comparable that wild-type mice. In addition, analysis several normally lack revealed reactivation Telomere length, even later mice, was equivalent animals. ES deficient also showed detectable alteration or telomere length increased passage culture. Thus, appears be completely dispensable vivo. Recently, has been identified within second ribonucleoprotein (RNP) complex, vault particle. can bind small RNA, vRNA, which particle unrelated sequence These results reveal an RNA binding not restricted complex plays redundant assembly localization RNP