Recruitment of the SWI-SNF Chromatin Remodeling Complex as a Mechanism of Gene Activation by the Glucocorticoid Receptor τ1 Activation Domain

• DOI: 10.1128/mcb.20.6.2004-2013.2000
• Publication Date: 2002-07-27T09:58:18Z
• Authors: Annika E. Wallberg; Kristen E. Neely; Ahmed H. Hassan; Jan-Åke Gustafsson; Jerry L. Workman; Anthony P. H. Wright
• Keywords: SWI/SNF; SMARCA4

The SWI-SNF complex has been shown to alter nucleosome conformation in an ATP-dependent manner, leading increased accessibility of nucleosomal DNA transcription factors. In this study, we show that the can potentiate activity glucocorticoid receptor (GR) through N-terminal transactivation domain, τ1, both yeast and mammalian cells. GR-τ1 directly interact with purified complex, mutations τ1 affect vivo also interaction SWI-SNF. Furthermore, stimulate τ1-driven from chromatin templates vitro. Taken together, these results support a model which GR recruit target promoters during glucocorticoid-dependent gene activation. We provide evidence SAGA complexes represent independent pathways τ1-mediated activation but play overlapping roles are able compensate for one another under some conditions.