Interpretation of X Chromosome Dose atSex-lethalRequires Non-E-Box Sites for the Basic Helix-Loop-Helix Proteins SISB and Daughterless

• DOI: 10.1128/mcb.21.5.1581-1592.2001
• Publication Date: 2002-07-27T10:03:53Z
• Authors: Dun Yang; Hong Lu; Yong Hong; Timothy M. Jinks; Patricia A. Estes; James W. Erickson
• Keywords: Male; 0301 basic medicine; Binding Sites; Base Sequence; Models, Genetic; Amino Acid Motifs; DNA Mutational Analysis; Helix-Loop-Helix Motifs; Molecular Sequence Data; DNA Footprinting; DNA-Binding Proteins; Evolution, Molecular; 03 medical and health sciences; Drosophila melanogaster; Basic Helix-Loop-Helix Transcription Factors; Animals; Deoxyribonuclease I; Drosophila Proteins; Female; Cells, Cultured; Conserved Sequence; Gene Deletion

For Drosophila melanogaster flies, sexual fate is determined by the X chromosome number. The basic helix-loop-helix protein product of the X-linked sisterlessB (sisB or scute) gene is a key indicator of the X dose and functions to activate the switch gene Sex-lethal (Sxl) in female (XX), but not in male (XY), embryos. Zygotically expressed sisB and maternal daughterless (da) proteins are known to form heterodimers that bind E-box sites and activate transcription. We examined SISB-Da binding at Sxl by using footprinting and gel mobility shift assays and found that SISB-Da binds numerous clustered sites in the establishment promoter Sxl(Pe). Surprisingly, most SISB-Da sites at Sxl(Pe) differ from the canonical CANNTG E-box motif. These noncanonical sites have 6-bp CA(G/C)CCG and 7-bp CA(G/C)CTTG cores and exhibit a range of binding affinities. We show that the noncanonical sites can mediate SISB-Da-activated transcription in cell culture. P-element transformation experiments show that these noncanonical sites are essential for Sxl(Pe) activity in embryos. Together with previous deletion analysis, the data suggest that the number, affinity, and position of SISB-Da sites may all be important for the operation of the Sxl(Pe) switch. Comparisons with other dose-sensitive promoters suggest that threshold responses to diverse biological signals have common molecular mechanisms, with important variations tailored to suit particular functional requirements.