Discoidin Domain Receptor 1 Tyrosine Kinase Has an Essential Role in Mammary Gland Development

• DOI: 10.1128/mcb.21.8.2906-2917.2001
• Publication Date: 2002-07-27T10:03:53Z
• Authors: Wolfgang F. Vogel; Attila Aszódi; Frauke Alves; Tony Pawson
• Keywords: DDR1; Discoidin domain

Various types of collagen have been identified as potential ligands for the two mammalian discoidin domain receptor tyrosine kinases, DDR1 and DDR2. Here, we used a recombinant fusion protein between extracellular alkaline phosphatase to detect specific binding sites during mouse development. Major DDR1-binding activity, indicative ligand expression, were found in skeletal bones, skin, urogenital tract. Ligand expression uterus implantation mammary gland pregnancy colocalized with receptor. The generation DDR1-null mice by gene targeting yielded homozygous mutant animals that viable but smaller size than control littermates. majority females unable bear offspring due lack proper blastocyst into uterine wall. When did occur, lactate. Histological analysis showed alveolar epithelium failed secrete milk proteins lumen gland. lactational defect appears be caused hyperproliferation abnormal branching ducts. These results suggest is key mediator stromal-epithelial interaction ductal morphogenesis