The Mnd1 Protein Forms a Complex with Hop2 To Promote Homologous Chromosome Pairing and Meiotic Double-Strand Break Repair

• DOI: 10.1128/mcb.22.9.3078-3088.2002
• Publication Date: 2002-07-27T10:09:01Z
• Authors: Hideo Tsubouchi; G. Shirleen Roeder
• Keywords: Synapsis

The hop2 mutant of Saccharomyces cerevisiae arrests in meiosis with extensive synaptonemal complex (SC) formation between nonhomologous chromosomes. A screen for multicopy suppressors a hop2-ts allele identified the MND1 gene. mnd1-null meiotic prophase, most double-strand breaks (DSBs) unrepaired. low level mature recombinants is produced, and Rad51 protein accumulates at numerous foci along SC incomplete, homolog pairing severely reduced. Mnd1 localizes to chromatin throughout this localization requires Hop2. Unlike recombination enzymes such as Rad51, chromosomes even mutants that fail initiate recombination. Hop2 proteins coimmunoprecipitate from cell extracts. These results suggest work promote chromosome DSB repair. identification homologs other organisms suggests function conserved among eukaryotes.