Two Different Drosophila ADA2 Homologues Are Present in Distinct GCN5 Histone Acetyltransferase-Containing Complexes

• DOI: 10.1128/mcb.23.1.306-321.2003
• Publication Date: 2002-12-13T21:43:47Z
• Authors: Selen Muratoglu; Sofia Georgieva; Gábor Pápai; Elisabeth Scheer; Izzet Enünlü; Orbán Komonyi; Imre Cserpán; Lubov Lebedeva; Elena Nabirochkina; Andor Udvardy; László Tora; Imre Boros
• Keywords: Histone acetyltransferase; Acetyltransferases

We have isolated a novel Drosophila (d) gene coding for two distinct proteins via alternative splicing: homologue of the yeast adaptor protein ADA2, dADA2a, and subunit RNA polymerase II (Pol II), dRPB4. Moreover, we identified another in genome encoding second ADA2 (dADA2b). The dADA2 homologues, as well many putative homologues from different species, all contain, addition to ZZ SANT domains, several evolutionarily conserved domains. dada2a/rpb4 dada2b genes are differentially expressed at various stages development. Both dADA2a dADA2b interacted with GCN5 histone acetyltransferase (HAT) two-hybrid assay, dADA2b, but not also ADA3. dADA2s further potentiate transcriptional activation insect mammalian cells. Antibodies raised either against or both immunoprecipitated TATA binding protein-associated factors (TAFs). following glycerol gradient sedimentation chromatographic purification combined gel filtration nuclear extracts, dGCN5 were detected fractions an apparent molecular mass about 0.8 MDa whereas was found corresponding masses least 2 MDa, together TAFs. Furthermore, vivo showed localizations on polytene X chromosomes. These results, taken together, suggest that present GCN5-containing HAT complexes.