Cited1 Is Required in Trophoblasts for Placental Development and for Embryo Growth and Survival

• DOI: 10.1128/mcb.24.1.228-244.2004
• Publication Date: 2003-12-12T20:13:07Z
• Authors: Tristan A. Rodriguez; Duncan B. Sparrow; Annabelle N. Scott; Sarah L. Withington; Jost I. Preis; Jan Michalicek; Melanie Clements; Tania E. Tsang; Toshi Shioda; Rosa S. P. Beddin...; Sally L. Dunwoodie
• Keywords: Null allele

Cited1 is a transcriptional cofactor that interacts with Smad4, estrogen receptors α and β, TFAP2, CBP/p300. It expressed in restricted manner the embryo as well extraembryonic tissues during embryonic development. In this study we report engineering of loss-of-function mutation mouse. null mutants show growth restriction at 18.5 days postcoitum, most them die shortly after birth. Half heterozygous females, i.e., those carry paternally inherited wild-type allele, are similarly affected. normally trophectoderm-derived cells placenta; however, these not cells. This occurs because located on X chromosome, thus allele paternal chromosome preferentially inactivated. Loss resulted abnormal placental mutants, spongiotrophoblast layer irregular shape enlarged while labyrinthine reduced size. addition, blood spaces within disrupted; maternal sinusoids considerably larger leading to reduction surface area available for nutrient exchange. We conclude required trophoblasts normal development subsequently viability.