Edd, the Murine Hyperplastic Disc Gene, Is Essential for Yolk Sac Vascularization and Chorioallantoic Fusion

• DOI: 10.1128/mcb.24.16.7225-7234.2004
• Publication Date: 2004-07-28T23:12:46Z
• Authors: Darren N. Saunders; Samantha L. Hird; Sarah L. Withington; Sally L. Dunwoodie; Michelle J. Hende...; Christine Biben; Robert L. Sutherland; Christopher J. Or...; Colin K. W. Watts
• Keywords: Conditional gene knockout

EDD is the mammalian ortholog of Drosophila melanogaster hyperplastic disc gene (hyd), which critical for cell proliferation and differentiation in flies through regulation hedgehog decapentaplegic signaling. Amplification overexpression occurs frequently several cancers, including those breast ovary, truncating mutations are also observed gastric colon cancer with microsatellite instability. has E3 ubiquitin ligase activity, involved DNA damage response, may control signaling, but a definitive biological role yet to be established. To investigate Edd vivo, targeting was used generate knockout (EddΔ/Δ) mice. While heterozygous mice had normal development fertility, no viable Edd-deficient embryos were beyond E10.5, delayed growth evident from E8.5 onward. Failed yolk sac allantoic vascular development, along defective chorioallantoic fusion, primary effects deficiency. These extraembryonic defects presumably compromised fetal-maternal circulation hence efficient exchange nutrients oxygen between embryo maternal environment, leading general failure embryonic widespread apoptosis. Hence, an essential development.