Product of Saccharomyces cerevisiae nuclear gene PET494 activates translation of a specific mitochondrial mRNA.

• DOI: 10.1128/mcb.6.11.3694
• Publication Date: 2015-09-30T19:39:02Z
• Authors: M C Costanzo; T D Fox
• Keywords: Nuclear gene

The product of Saccharomyces cerevisiae nuclear gene PET494 is known to be required for a posttranscriptional step in the accumulation one mitochondrial product, subunit III cytochrome c oxidase (coxIII). Here we show that protein probably acts mitochondria by demonstrating both PET494-beta-galactosidase fusion and unmodified are specifically associated with mitochondria. To define site action, isolated mutations suppress pet494 deletion. These were rearrangements oxi2 encodes coxIII. suppressor genes had acquired 5'-flanking sequences other gave rise transcripts carrying 5'-untranslated leaders their mRNAs. results demonstrate wild-type cells promotes coxIII translation, interacting leader mRNA.