Genetic evidence for promoter competition in Saccharomyces cerevisiae.

• DOI: 10.1128/mcb.8.11.4608
• Publication Date: 2015-09-30T19:48:44Z
• Authors: J E Hirschman; K J Durbin; F Winston
• Keywords: Transcription; Retrotransposon

The his4-912 delta mutation is an insertion of the long terminal repeat (delta) yeast retrotransposon Ty into HIS4 promoter region, such that 97 base pairs upstream transcription initiation site. Strains carrying allele are His- at 23 degrees C; this phenotype can be reversed either by growth 37 C or mutations in trans-acting SPT genes. Under conditions which confers a phenotype. initiates site, rather than producing longer, nonfunctional transcript. strain His+, wild-type To understand how balanced between and promoters, we have selected for cis-acting suppressors delta. Two classes defined six independent restore synthesis functional first class A-to-G change 1 proposed TATA sequence. These mutants do not synthesize delta-initiated transcript; instead, they only second alters surrounding two strongest His+ transcript levels consistent with their Surprisingly, these also reduced level relative to parent. Analysis indicates from one affect other suggests signals compete each