Infection with the intracellular protozoan parasite Theileria parva induces constitutively high levels of NF-kappa B in bovine T lymphocytes.

• DOI: 10.1128/mcb.9.11.4677
• Publication Date: 2015-10-01T21:49:03Z
• Authors: V Ivanov; B Stein; I Baumann; D A Dobbelaere; P Herrlich; R O Williams
• Keywords: Kappa; Theileria parva

The intracellular protozoan parasite Theileria parva causes a lymphoproliferative disease of T cells in cattle and uncontrolled lymphocyte proliferation culture. We have identified characterized infected the transcriptional activator, NF-kappa B, whose recognition motifs been several gene enhancers important for lymphocyte-specific expression. B is normally constitutively activated nuclear extracts derived from can be induced nonlymphoid by phorbol esters. Theileria-infected lymphocytes contained high levels fractions inactive cytoplasmic fractions. precursor could treatment with deoxycholate, which was shown previously to dissociate an inhibitor, I kappa B. Treatment 3 mM GTP stimulated binding its motif vitro, thereby distinguishing it related factor, H2-TF1. Selective killing parasite, left host intact, resulted rapid loss slower In parasitized cells, not further 12-O-tetradecanoylphorbol-13-acetate whereas treated remove this compound elevated propose that are maintained presence cells. Similarly, we result increased synthesis due parasite.