Interlaboratory Comparison of the Pneumococcal Multiplex Opsonophagocytic Assays and Their Level of Agreement for Determination of Antibody Function in Pediatric Sera

• DOI: 10.1128/msphere.00070-18
• Publication Date: 2018-04-24T09:19:44Z
• Authors: A. Balloch; L. Roalfe; N. Ekstrom; C. D. Nguyen; L. Spry; R. A. Marimla; P. V. Licciardi; D. Goldblatt; E. K. Mulholland
• Keywords: 0301 basic medicine; Heptavalent Pneumococcal Conjugate Vaccine; 610; Immunologic Tests; immunization; Serogroup; World Health Organization; Microbiology; Pneumococcal Infections; 618; Pneumococcal Vaccines; 03 medical and health sciences; Immunogenicity, Vaccine; Phagocytosis; Humans; Child; Antigens, Bacterial; 0303 health sciences; Reproducibility of Results; MOPA; Opsonin Proteins; Antibodies, Bacterial; QR1-502; pneumococcal IgG; 3. Good health; Streptococcus pneumoniae; Child, Preschool; Immunoglobulin G; Research Article

When measuring a functional antibody response to pneumococcal immunization, it is imperative that a specific, reproducible, accurate, and standardized assay with acceptable inter- and intra-assay variation be advocated internationally to allow for meaningful comparison of results between laboratories. We report here the results of a collaboration between 3 international laboratories testing 30 pediatric samples against the 13 serotypes in Prevenar13.