Enzymatically enhanced ultrastructure expansion microscopy unlocks expansion of in vitro Toxoplasma gondii cysts

• DOI: 10.1128/msphere.00322-24
• Publication Date: 2024-08-27T13:01:36Z
• Authors: Kseniia Bondarenko; Floriane Limoge; Kayvon Pedram; Mathieu Gissot; Joanna C. Young
• Keywords: Neurons; 0301 basic medicine; Microscopy; [SDV]Life Sciences [q-bio]; Protozoan Proteins; host–parasite relationship; Fibroblasts; chronic infection; Microbiology; QR1-502; Mice; 03 medical and health sciences; apicomplexan parasites; Animals; expansion microscopy; Toxoplasma; Toxoplasmosis; Research Article

Expansion microscopy (ExM) is an innovative approach to achieve super-resolution images without using microscopes, based on the physical expansion of sample. The advent ExM has unlocked detail for a broader scientific circle, lowering cost and entry skill requirements field. One its branches, ultrastructure (U-ExM), become popular among research groups studying apicomplexan parasites, including acute stage