Identification of Novel Host Interactors of Effectors Secreted by Salmonella and Citrobacter

• DOI: 10.1128/msystems.00032-15
• Publication Date: 2016-07-13T02:13:21Z
• Authors: Ryan L. Sontag; Ernesto S. Nakayasu; Roslyn N. Brown; George S. Niemann; Michael A. Sydor; Octavio Sanchez; Charles Ansong; Shao-Yeh Lu; Hyungwon Choi; Dylan Valleau; Karl K. Weitz; Alexei Savchenko; Eric D. Cambronne; Joshua N. Adkins
• Keywords: 0303 health sciences; 03 medical and health sciences; protein-protein interactions; affinity purification; pathogenic bacteria; Microbiology; QR1-502; mass spectrometry; effectors; type III secretion system; Research Article; 3. Good health

During infection, pathogenic bacteria face an adverse environment of factors driven by both cellular and humoral defense mechanisms. To help evade the immune response and ultimately proliferate inside the host, many bacteria evolved specialized secretion systems to deliver effector proteins directly into host cells. Translocated effector proteins function to subvert host defense mechanisms. Numerous pathogenic bacteria use a specialized secretion system called type III secretion to deliver effectors into the host cell cytosol. Here, we identified 75 new host targets of Salmonella and Citrobacter effectors, which will help elucidate their mechanisms of action.