The Entner-Doudoroff and Nonoxidative Pentose Phosphate Pathways Bypass Glycolysis and the Oxidative Pentose Phosphate Pathway in Ralstonia solanacearum

• DOI: 10.1128/msystems.00091-20
• Publication Date: 2020-03-09T09:55:39Z
• Authors: Poonam Jyoti; Manu Shree; Chandrakant Joshi; Tulika Prakash; Suvendra Kumar Ray; Siddhartha Sankar...; Shyam Kumar Masak...
• Keywords: Ralstonia solanacearum; Metabolic pathway; Dihydroxyacetone phosphate

In Ralstonia solanacearum, a devastating phytopathogen whose metabolism is poorly understood, we observed that the Entner-Doudoroff (ED) pathway and nonoxidative pentose phosphate (non-OxPPP) bypass glycolysis OxPPP under glucose oxidation. Evidence derived from 13C stable isotope feeding genome annotation-based comparative metabolic network analysis supported observations. Comparative currently available 53 annotated R. solanacearum strains, including recently reported strain (F1C1), representing four phylotypes, confirmed lack of key genes coding for phosphofructokinase (pfk-1) phosphogluconate dehydrogenase (gnd) enzymes are relevant OxPPP, respectively. F1C1 cells fed with [13C]glucose (99% [1-13C]glucose or 99% [1,2-13C]glucose 40% [13C6]glucose) followed by gas chromatography-mass spectrometry (GC-MS)-based labeling fragments amino acids, glycerol, ribose provided clear evidence rather than ED non-OxPPP main routes sustaining in The incorporation mass ions alanine (m/z 260 m/z 232), valine 288 260), glycine 218), serine 390 362), histidine 440 412), tyrosine 466 438), phenylalanine 336 308), glycerol 377), 160) mapped pathways supporting outcomes help better define central carbon can be integrated flux as well balance studies defining phenotypes.IMPORTANCE Understanding versatility important, it regulates trade-off between virulence (1, 2) wide range plant hosts. Due to until this work, several published research papers on potential roles oxidative (OxPPP) (3, 4). This work during oxidation glucose, component host xylem pool serves source (5). phenotypes. study highlights need critically examine phytopathogens understood.