Novel isolates of hydrogen-oxidizing chemolithoautotrophic Sulfurospirillum provide insight to the functions and adaptation mechanisms of Campylobacteria in shallow-water hydrothermal vents

• DOI: 10.1128/msystems.00148-24
• Publication Date: 2024-08-22T01:02:24Z
• Authors: Li Wang; Xinyi Cheng; Yizhe Guo; Junwei Cao; Mingye Sun; Jiang-Shiou Hwang; Rulong Liu; Jiasong Fang
• Keywords: Chemosynthesis; Microbial mat

ABSTRACT Enhancing the availability of representative isolates from hydrothermal vents (HTVs) is imperative for comprehending microbial processes that propel vent ecosystem. In recent years, Campylobacteria have emerged as predominant and ubiquitous taxon across both shallow deep-sea systems. Nevertheless, only a few been cultured, primarily originating HTVs. Presently, no cultivable are accessible in water systems (<200 m), which exhibit markedly distinct environmental conditions their counterparts. this study, we enriched novel isolate (genus Sulfurospirillum , Campylobacteria) shallow-water HTVs Kueishan Island. Genomic physiological analysis revealed species grows on variety substrate carbon/energy sources. The pan-genome phenotypic comparisons with 12 previously isolated different environments supported identification functional features genomes crucial adaptation to environments, such sulfur oxidation, carbon fixation, biofilm formation, benzoate/toluene degradation, well diverse genes related signal transportation. To conclude, metabolic characteristics augment our understanding spanning IMPORTANCE emerge dominant taxa within systems, playing important roles ecosystems. However, representatives mainly fields, leaving significant knowledge gap regarding functions, activities, strategies microorganisms (HTVs). This study bridges by providing insights into phenomics genomic diversity genus (order Campylobacterales, class based data derived obtained Our mesophilic not augments pure cultures but also serves inaugural reference environments.