Redirected Stress Responses in a Genome-Minimized ‘midi Bacillus ’ Strain with Enhanced Capacity for Protein Secretion

• DOI: 10.1128/msystems.00655-21
• Publication Date: 2021-12-14T14:30:21Z
• Authors: Rocío Aguilar Suárez; Minia Antelo-Varela; Sandra Maaß; Jolanda Neef; Dörte Becher; Jan Maarten van Dijl
• Keywords: Proteome; Secretory protein; Bacterial genome size

Genome engineering offers the possibility to create completely novel cell factories with enhanced properties for biotechnological applications. In recent years, genome minimization was extensively explored in Gram-positive bacterial factory Bacillus subtilis, where up 42% of encoding dispensable functions removed. Such studies showed that some strains minimized genomes gained beneficial features, especially secretory protein production. However, most minimal displayed growth defects. This focused our attention on less extensive genomic deletions display close-to-wild-type while retaining acquired traits A strain this category is B. subtilis IIG-Bs27-47-24, here referred as midiBacillus, which lacks 30.95% parental genome. To date, it unknown how altered configuration midiBacillus impacts physiology general, and secretion particular. The present study bridges knowledge gap through comparative quantitative proteome analyses focus secretion. Interestingly, results show stress responses elicited by high-level expression immunodominant staphylococcal antigen A, are different from occur 168. We further has an increased capacity translation a variety critical Sec machinery components at elevated levels. Altogether, observations demonstrate consequences wild-type genome-engineered strains, dictated proteomic configurations. IMPORTANCE Our showcases genome-minimized nonpathogenic bacterium, so-called chassis development future industrial serve production high-value difficult-to-produce proteins. particular, we explain about one-third original genome, effectively secretes major human pathogen Staphylococcus aureus cannot be produced strain. important, because secreted S. exemplary range targets can implemented antistaphylococcal immunotherapies. Accordingly, anticipate will contribute vaccines protect both humans livestock against diseases caused aureus, increasingly difficult fight antibiotics, accumulated resistances essentially all antibiotics currently clinical practice.