A High-Throughput Method for Identifying Novel Genes That Influence Metabolic Pathways Reveals New Iron and Heme Regulation in Pseudomonas aeruginosa

• DOI: 10.1128/msystems.00933-20
• Publication Date: 2021-02-08T20:38:47Z
• Authors: David G. Glanville; Caroline Mullinea...; Christopher J. Co...; Brian T. Burger; Saheed Imam; Timothy J. Donohue; Andrew T. Ulijasz
• Keywords: 0301 basic medicine; Tn-seq; heme biosynthesis; heme transport; biosensor; Microbiology; QR1-502; 3. Good health; 03 medical and health sciences; Pseudomonas aeruginosa; heme; Research Article

The ability to simultaneously and more directly correlate genes with metabolite levels on a global level would provide novel information for many biological platforms yet has thus far been challenging. Here, we describe a method to help address this problem, which we dub “Met-Seq” ( met abolite-coupled Tn seq uencing).