Multi-omics strategy reveals potential role of antimicrobial resistance and virulence factor genes responsible for Simmental diarrheic calves caused by Escherichia coli

• DOI: 10.1128/msystems.01348-23
• Publication Date: 2024-05-14T13:00:27Z
• Authors: Zhihai Shi; Yali Lan; Yazhou Wang; Xiangzhou Yan; Xiaoya Ma; Faiz-ul Hassan; Hossam E. Rushdi; Zhaoxue Xu; Wenjia Wang; Tingxian Deng
• Keywords: Diarrhea; Virulence Factors; Epidemiology; Antibiotic resistance; diarrhea; Cattle Diseases; Microbiology; Gene; metagenome; Diagnosis, Treatment, and Epidemiology of Nontuberculous Mycobacterial Diseases; antibiotic resistance genes; Antibiotics; Biochemistry, Genetics and Molecular Biology; Drug Resistance, Bacterial; Health Sciences; Escherichia coli; Genetics; Animals; Metabolomics; Molecular Biology; Biology; Internal medicine; Escherichia coli Infections; virulence factor genes; Ecology; Obesity-associated Microbiome; Virulence; Life Sciences; Virulence factor; Multiomics; Diversity and Function of Gut Microbiome; Ecology and Evolution of Viruses in Ecosystems; metabolomics; QR1-502; Gastrointestinal Microbiome; Anti-Bacterial Agents; FOS: Biological sciences; Environmental Science; Physical Sciences; Medicine; Cattle; Research Article

ABSTRACT

Escherichia coli ( E. coli ) is reported to be an important pathogen associated with calf diarrhea. Antibiotic resistance genes (ARGs) and virulence factor genes (VFGs) pose a considerable threat to both animal and human health. However, little is known about the characterization of ARGs and VFGs presented in the gut microbiota of diarrheic calves caused by E. coli . In this study, we used multi-omics strategy to analyze the ARG and VFG profiles of Simmental calves with diarrhea caused by E. coli K99. We found that gut bacterial composition and their microbiome metabolic functions varied greatly in diarrheic calves compared to healthy calves. In total, 175 ARGs were identified, and diarrheal calves showed a significantly higher diversity and abundance of ARGs than healthy calves. Simmental calves with diarrhea showed higher association of VFGs with pili function, curli assembly, and ferrienterobactin transport of E. coli . Co-occurrence patterns based on Pearson correlation analysis revealed that E. coli had a highly significant ( P < 0.0001) correlation coefficient (>0.8) with 16 ARGs and 7 VFGs. Metabolomics analysis showed that differentially expressed metabolites in Simmental calves with diarrhea displayed a high correlation with the aforementioned ARGs and VFGs. Phylotype analysis of E. coli genomes showed that the predominant phylogroup B1 in diarrheic Simmental calves was associated with 10 ARGs and 3 VFGs. These findings provide an overview of the diversity and abundance of the gut microbiota in diarrheic calves caused by E. coli and pave the way for further studies on the mechanisms of antibiotic resistance and virulence in the calves affected with diarrhea.

IMPORTANCE

Simmental is a well-recognized beef cattle breed worldwide. They also suffer significant economic losses due to diarrhea. In this study, fecal metagenomic analysis was applied to characterize the antibiotic resistance gene (ARG) and virulence factor gene (VFG) profiles of diarrheic Simmental calves. We identified key ARGs and VFGs correlated with Escherichia coli isolated from Simmental calves. Additionally, metabolomics analysis showed that differentially expressed metabolites in Simmental calves with diarrhea displayed a high correlation with the aforementioned ARGs and VFGs. Our findings provide an insight into the diversity and abundance of the gut microbiota in diarrheic calves caused by Escherichia coli and pave the way for further studies on the mechanisms of antibiotic resistance and virulence in the diarrheal calves from cattle hosts.