Hijacking of Host Plasminogen by Mesomycoplasma ( Mycoplasma ) hyopneumoniae via GAPDH: an Important Virulence Mechanism To Promote Adhesion and Extracellular Matrix Degradation

• DOI: 10.1128/spectrum.00218-23
• Publication Date: 2023-05-18T14:03:25Z
• Authors: Jiying Wang; Shiyang Li; Junhong Chen; Lanxi Gan; Jia Wang; Qiyan Xiong; Zhixin Feng; Quan Li; Zhibang Deng; Xiaomin Yuan; Yanfei Yu
• Keywords: Mycoplasma hyopneumoniae

Mesomycoplasma hyopneumoniae is the etiological agent of mycoplasmal pneumonia swine (MPS), which causes substantial economic losses to world's industry. Moonlighting proteins are increasingly being shown play a role in pathogenic process M. hyopneumoniae. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), key enzyme glycolysis, displayed higher abundance highly virulent strain than an attenuated strain, suggesting that it may have virulence. The mechanism by GAPDH exerts its function was explored. Flow cytometry and colony blot analysis showed partly on surface Recombinant (rGAPDH) able bind PK15 cells, while adherence mycoplasma significantly blocked anti-rGAPDH antibody pretreatment. In addition, rGAPDH could interact with plasminogen. rGAPDH-bound plasminogen demonstrated be activated plasmin, as proven using chromogenic substrate, further degrade extracellular matrix (ECM). critical site for binding K336, amino acid mutation. affinity C-terminal mutant (K336A) decreased according plasmon resonance analysis. Collectively, our data suggested might important virulence factor facilitates dissemination hijacking host tissue ECM barrier. IMPORTANCE specific pathogen pigs responsible industry worldwide. pathogenicity possible particular determinants not yet completely elucidated. Our suggest (ECM) These findings will provide theoretical support new ideas research development live-attenuated or subunit vaccines against