Two Novel Adenovirus Vectors Mediated Differential Antibody Responses via Interferon-α and Natural Killer Cells

• DOI: 10.1128/spectrum.00880-23
• Publication Date: 2023-06-22T18:47:56Z
• Authors: Peng Zou; Panli Zhang; Qitao Deng; Cong Wang; Shengxue Luo; Ling Zhang; Chengyao Li; Tingting Li
• Keywords: COVID-19 Vaccines; SARS-CoV-2; Adenoviruses, Human; Genetic Vectors; Interferon-alpha; COVID-19; antibody response; NK cells; Microbiology; QR1-502; 3. Good health; Adenoviridae; Killer Cells, Natural; Mice; interferon-α; adenoviruses; Antibody Formation; Humans; Animals; Adenoviruses, Simian; novel adenovirus vector; antibody function; Research Article

Recombinant adenovirus vectors have been widely used in vaccine development. To overcome the preexisting immunity of human type 5 (Ad5) populations, a range chimpanzee or rare generated. However, these novel mediate diverse immune responses hosts. In this study, we explored mechanism differential antibody to SARS-CoV-2 S protein mice immunized by our previously developed two simian 23 (Sad23L) and 49 (Ad49L), Ad5 vectored COVID-19 vaccines. Sad23L-nCoV-S Ad5-nCoV-S vaccines induced low level interferon-α (IFN-α) high antigen-specific wild-type IFN-α/β receptor defective (IFNAR-/-) C57 mice, while Ad49L-nCoV-S IFN-α but response IFNAR-/- mice. addition, was detected natural killer (NK) cells-blocked follicular helper T (TFH) cells -blocked with vaccine. These results showed that Ad49L stimulated secretion activate NK cells, then reduced number TFH generation center (GC) B plasma subsequently production. The different could be selected for development according need either humoral cellular both protections against particular disease. IMPORTANCE Novel are an important antigen delivery platform Understanding diversity between adenoviral is critical design proper aim described Sad23L raising equally specific cell We found Ad49L-vectored initiated activated inhibit via downregulating CD4+ leading decline GC cells.