CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern

• DOI: 10.1128/spectrum.01017-21
• Publication Date: 2021-11-17T15:32:53Z
• Authors: Yuanhao Liang; Hongqing Lin; Lirong Zou; Jianhui Zhao; Baisheng Li; Haiying Wang; Jing Lu; Jiufeng Sun; Xingfen Yang; Xiaoling Deng; Shixing Tang
• Keywords: 0301 basic medicine; SARS-CoV-2; COVID-19; Microbiology; Polymerase Chain Reaction; QR1-502; 3. Good health; 03 medical and health sciences; COVID-19 Testing; CRISPR-Cas system; Mutation; Spike Glycoprotein, Coronavirus; emerging SARS-CoV-2 variant; Humans; Mass Screening; Public Health; CRISPR-Cas Systems; Databases, Nucleic Acid; variant screening; Alleles; Research Article

We described CRISPR-Cas12-based multiplex allele-specific assay for rapid SARS-CoV-2 variant genotyping. The new system has the potential to be quickly developed, continuously updated, and easily implemented for screening of SARS-CoV-2 variants in resource-limited settings. This approach can be adapted for emerging mutations and implemented in laboratories already conducting SARS-CoV-2 nucleic acid amplification tests using existing resources and extracted nucleic acid.