Genomic Characterization of Antimicrobial-Resistant Salmonella enterica in Duck, Chicken, and Pig Farms and Retail Markets in Eastern China

• DOI: 10.1128/spectrum.01257-22
• Publication Date: 2022-09-01T13:01:52Z
• Authors: Biao Tang; Mohammed Elbediwi; Reshma B. Nambiar; Hua Yang; Jiahui Lin; Min Yue
• Keywords: Salmonella enterica; Fosfomycin; Gene cassette; Resistome

Antimicrobial-resistant Salmonella enterica poses a significant public health concern worldwide. However, the dissemination of among food animals in eastern China has not been fully addressed. Here, we demonstrated antimicrobial resistance (AMR) patterns and whole-genome characterization 105 S. isolates from 1,480 fecal samples anal swabs collected 22 different farms (chickens, ducks, pigs) two live animal markets located Zhejiang Fujian Provinces 2019. The prevalence duck (19.17%, 23/120) was statistically significantly higher (P < 0.001) than that chicken (6.61%, 37/523) pig (3.50%, 7/200). Among these isolates, 75.26% (79/105) were multidrug resistant, with highest rates to tetracycline (76.20%) ampicillin (67.62%) lowest rate meropenem (0.00%). serotypes consistent sequence types closely related sampling species sites. serotype Kentucky (20.95%, 22/105) most frequent harbored more AMR genes others. Furthermore, IncFII(S) IncHI2 prevalent replicons. A total 44 acquired found. those genes, aac(6')-Iaa, blaTEM-1B, floR, dfrA14, fosA7, mph(A), qnrS1, sul1, tet(A), ARR-3 dominant mediating toward aminoglycosides, β-lactams, phenicol, trimethoprim, fosfomycin, macrolide, quinolone, sulfonamides, tetracycline, rifampin, respectively. consistency phenotypes for ampicillin, ceftiofur, ceftazidime, meropenem, sulfamethoxazole-trimethoprim, >90%. Together, our study highlights application sequencing assess veterinary threats. IMPORTANCE Public is China, foodborne pathogen Salmonella, which spread via animal-borne chain, plays an important role overall disease burden annually. development advanced technologies introduced new way understanding emerging pathogens. routine surveillance this method remains its infancy. applied pool all data combined cutting-edge bioinformatic analysis pipeline one-step determination complete genetic makeup genomes. illustrated could provide cost-effective approach, without labor-intensive laboratory characterization, predicting serotypes, genotypes, plasmid types, virulence thus would essential knowledge Our findings perspectives are delivering updated on pathogens understudied region China.