GpsB Coordinates Cell Division and Cell Surface Decoration by Wall Teichoic Acids in Staphylococcus aureus

• DOI: 10.1128/spectrum.01413-22
• Publication Date: 2022-06-01T13:57:00Z
• Authors: Lauren R. Hammond; Michael D. Sacco; Sebastian J. Khan; Catherine Spanoudis; Abigail Hough-Neidig; Yu Chen; Prahathees J. Eswara
• Keywords: Teichoic acid; FtsZ

Bacterial cell division is a complex and highly regulated process requiring the coordination of many different proteins. Despite substantial work in model organisms, our understanding systems regulating noncanonical including critical human pathogens, far from complete. One such organism Staphylococcus aureus, spherical bacterium that lacks known regulatory Recent studies on GpsB, protein conserved within Firmicutes phylum, have provided insight into regulation S. aureus other related organisms. It has been revealed GpsB coordinates wall synthesis multiple species. In we previously shown directly regulates FtsZ polymerization. this study, using Bacillus subtilis as tool, isolated spontaneous suppressors abrogate lethality overproduction B. subtilis. Through characterization, identified several residues important for function GpsB. Furthermore, discovered an additional role teichoic acid (WTA) biosynthesis aureus. Specifically, show interacts with WTA export TarG. We also region crucial interaction. Analysis TarG localization suggests machinery part divisome complex. Taken together, research illustrates how performs essential by linking tightly cycle processes WTA-mediated surface decoration. IMPORTANCE Cytokinesis bacteria involves intricate orchestration key proteins factors involved building robust envelope. Presence acids signature characteristic Gram-positive wall. By characterizing organism, uncovered biosynthesis. may be homologs exporter follow similar patterns. suggested acts molecular signal to control activity autolytic enzymes, especially during separation conjoined daughter cells. Thus, results reveal addition playing division, help coordinate biogenesis.