Evaluation of Sample Preservation and Storage Methods for Metaproteomics Analysis of Intestinal Microbiomes

• DOI: 10.1128/spectrum.01877-21
• Publication Date: 2021-12-15T15:31:48Z
• Authors: Angie Mordant; Manuel Kleiner
• Keywords: Male; Proteomics; 0301 basic medicine; Preservation, Biological; Microbiology; Feces; Mice; 03 medical and health sciences; Bacterial Proteins; Tandem Mass Spectrometry; microbiota; Animals; LC-MS/MS; 0303 health sciences; Bacteria; intestinal microbiome; QR1-502; Gastrointestinal Microbiome; gut microbes; Mice, Inbred C57BL; metaproteomics; Female; Peptides; sample preservation; Research Article; Chromatography, Liquid

Metaproteomics is a powerful tool to study the intestinal microbiome. By identifying and quantifying a large number of microbial, dietary, and host proteins in microbiome samples, metaproteomics provides direct evidence of the activities and functions of microbial community members.