Sensitivity and Specificity of SARS-CoV-2 Rapid Antigen Detection Tests Using Oral, Anterior Nasal, and Nasopharyngeal Swabs: a Diagnostic Accuracy Study

• DOI: 10.1128/spectrum.02029-21
• Publication Date: 2022-02-02T15:31:07Z
• Authors: Michael Wölfl-Duchek; Felix Bergmann; Anselm Jorda; Maria Weber; Matthias Müller; Tamara Seitz; Alexander Zoufaly; Robert Strassl; Markus Zeitlinger; Harald Herkner; Harald Schnidar; Karolina Anderle; Ulla Derhaschnig
• Keywords: Adult; Male; infectious disease; specificity; Nose; Microbiology; Polymerase Chain Reaction; Sensitivity and Specificity; COVID-19 Serological Testing; Young Adult; 03 medical and health sciences; rapid antigen detection; 0302 clinical medicine; Nasopharynx; Humans; Prospective Studies; Antigens, Viral; Aged; Aged, 80 and over; Mouth; SARS-CoV-2; COVID-19; Middle Aged; sensitivity; 16. Peace & justice; QR1-502; 3. Good health; PCR; COVID-19 Nucleic Acid Testing; Female; Research Article

The objective of our study was to evaluate the sensitivity and specificity rapid antigen detection tests versus those reverse transcriptase PCR (RT-PCR) using oral, anterior nasal, nasopharyngeal swabs. underlying prospective, diagnostic case-control-type accuracy included 87 hospitalized nonhospitalized participants in a positive negative sample cohort between 16 March 14 May 2021 two hospitals Vienna. SARS-CoV-2 infection status confirmed by RT-PCR. Participants self-performed one oral nasal swab for test, immediately followed swabs test RT-PCR investigator. Test results were read after 15 min, completed questionnaire meantime. parameters calculated based on evaluation participants. overall that with samples 18.18% (95% confidence interval [CI] 8.19% 32.71%), 63.04% CI 47.55% 76.79%), 73.33% 58.06% 85.4%), respectively. All sampling methods had 100% regardless cycle threshold (