Antimicrobial and anti-biofilm activity of a thiazolidinone derivative against Staphylococcus aureus in vitro and in vivo

• DOI: 10.1128/spectrum.02327-23
• Publication Date: 2024-02-08T14:01:26Z
• Authors: Rui Zhao; Bingyu Du; Yue Luo; Fen Xue; Huanhuan Wang; Di Qu; Shiqing Han; Simon Heilbronner; Yanfeng Zhao
• Keywords: 570; Staphylococcus aureus; anti-biofilm activity; 610; WalK; bacteriocidal; Microbiology; QR1-502; Research Article

ABSTRACT Staphylococcus aureus ( S. ) causes many infections with significant morbidity and mortality. can form biofilms, which cause biofilm-associated diseases increase resistance to conventional antibiotics, resulting in chronic infection. It is critical develop novel antibiotics against staphylococcal infections, particularly those that kill cells embedded biofilms. This study aimed investigate the bacteriocidal anti-biofilm activities of thiazolidinone derivative (TD-H2-A) . A total 40 non-duplicate strains were collected, minimum inhibitory concentrations (MICs) TD-H2-A determined. The effect on established mature biofilms was examined using a confocal laser scanning microscope (CLSM). antibacterial effects compound planktonic bacteria investigated. Other characteristics, such as cytotoxicity hemolytic activity, researched. mouse skin infection model used, routine hematoxylin eosin (H&E) staining used for histological examination. MIC values different 6.3–25.0 µg/mL. 5 × killed almost all USA300. found have strong activity meanwhile having low Vero human erythrocytes. had good SA113-infected mice. In conclusion, demonstrated , paving way development agents combat biofilm multidrug-resistant infections. IMPORTANCE notorious pathogen, stubborn drug resistance. crucial new anti-infective therapies manuscript describes antibiotic effectively diseases.