Exploring functional microbiota for uranium sequestration in Zoige uranium mine soil

• DOI: 10.1128/spectrum.02517-24
• Publication Date: 2025-04-16T13:03:10Z
• Authors: Xiang Wang; Li Zhao; Xu Zhang; Yanxia Wei; Aixia Lu; Jian Zhou; Guiqiang He
• Keywords: Acidobacteria; Verrucomicrobia; Revegetation

ABSTRACT The Zoige uranium mine is situated in the harsh, cold northern region of Sichuan, characterized by its high altitude and fragile ecosystem. Uncovering organisms that thrive such extreme climates, particularly microorganisms, paramount importance for advancing bioremediation efforts. Herein, potential functional microbiota sequestration soil was explored using high-throughput sequencing combined with bioinformatics analysis. Analysis physicochemical properties soils showed concentration ranged from 35.20 to 40.62 µg·g −1 around mine. Bacterial communities differed significantly mine, most abundant phyla being Actinobacteria, Proteobacteria, Acidobacteria, Chloroflexi, Gemmatimonadota, Verrucomicrobia, Firmicutes. Notably, Actinobacteria considered a biomarker distinguishing linear discriminant analysis effect size. Meanwhile, correlation demonstrated Firmicutes Cyanobacteria were positively associated samples, coefficients 0.8601 0.7832, respectively. Furthermore, phylogenetic investigation reconstruction unobserved states revealed bacterial mainly enriched biosynthesis function these soils. Interestingly, abundance genes involved amino acid increased whereas related fatty decreased an increase content. Taken together, Firmicutes, via pathways soil. These findings are conducive obtaining strains developing microbial remediation technologies contamination. IMPORTANCE Based on significance unique ecological environment, this study emphasizes necessity situ bioremediation. microbes interacted tolerated pathways. provide insights into sequestration, which resources technology treating