Gut Microbiota Eubacterium callanderi Exerts Anti-Colorectal Cancer Activity

• DOI: 10.1128/spectrum.02531-22
• Publication Date: 2022-11-30T14:00:57Z
• Authors: Seoung Woo Ryu; Ji-Sun Kim; Byeong Seob Oh; Won Jung Choi; Seung Yeob Yu; Jeong Eun Bak; Seung-Hwan Park; Se Won Kang; Jiyoung Lee; Won Yong Jung; Jung-Sook Lee; Ju Huck Lee
• Keywords: Eubacterium

The gut microbiota (GM) is associated with colorectal cancer (CRC) development. However, studies demonstrating the role of GM in CRC are limited to metagenomic analyses. These lack direct evidence proving that candidate strains involved CRC, and isolated probiotics for bacteriotherapy. Therefore, identify novel anti-CRC activity, we previously bacteria from feces healthy individuals, screened GM's discovered cell-free supernatants isolates demonstrated antiproliferative activity against cells. Here, our study identified one them as Eubacterium callanderi chose it further because genus has been suggested contribute various aspects health; however, functions unknown. First, confirmed E. supernatant (EcCFS) exerted activity-by inducing apoptosis cell cycle arrest-that was dose-dependent specific lines. Next, EcCFS active molecules were heat stable protease insensitive. High-performance liquid chromatography analysis revealed contained high butyrate concentrations possessing anticancer activity. Additionally, gas chromatography-mass spectrometry aqueous phase ethyl acetate-extracted an antiproliferation assay 4-aminobutanoic acid (GABA) GABA a possible agent. Finally, CT26 allograft mouse model, oral administration peri-tumoral injection inhibited tumor growth vivo. reveals effect suggests may be potential developing control CRC. IMPORTANCE reported cancer, by analysis. limitations, such bias absence bacterial resources follow-up studies. attempted discover microorganisms related using culturomics method. In this study, possesses anti-colorectal vitro vivo, suggesting could used bacteriotherapy treatment.