A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum

• DOI: 10.1128/spectrum.02782-21
• Publication Date: 2022-05-05T13:00:56Z
• Authors: Xiaoying Liang; Rachasak Boonhok; Faiza Amber Siddiqui; Bo Xiao; Xiaolian Li; Junling Qin; Hui Min; Lubin Jiang; Liwang Cui; Jun Miao
• Keywords: 0301 basic medicine; Resource Report; Plasmodium falciparum; Microbiology; QR1-502; Artemisinins; Epigenesis, Genetic; 3. Good health; dCas9; Antimalarials; 03 medical and health sciences; Gene Expression Regulation; DiCre; CRISPR-Cas Systems; gene regulation; human malaria

Understanding the fundamental biology of malaria parasites through functional genetic/genomic studies is critical for identifying novel targets for antimalarial development. Conditional knockout/knockdown systems are required to study essential genes in the haploid blood stages of the parasite.