In vitro dynamics of rumen microbiota and fermentation profiles with Antler growth of Sika deer

• DOI: 10.1128/spectrum.02829-24
• Publication Date: 2025-01-28T14:02:11Z
• Authors: Songze Li; Shaoying Wang; Yuhang Zhu; Ruina Mu; Tao Wang; Yuguo Zhen; Huazhe Si; Rui Du; Zhipeng Li
• Keywords: in vitro fermentation; rumen microbiota; antler growth; butyrate; Microbiology; QR1-502

ABSTRACT

The rumen microbiota plays a vital role in the nutrient metabolism affecting the growth of velvet antler. However, the fermentation patterns and dynamics of the rumen microbiota across growth stages of velvet antler remain largely unexplored. Here, we employed an in vitro fermentation approach to assess fermentation parameters and microbial composition in the rumen liquid of sika deer during the early growth (EG), metaphase growth (MG), and fast growth (FG) phases . Our findings indicated that the levels of short-chain fatty acids (SCFAs), ammonia nitrogen, and gas production increased over fermentation time in all three groups. The concentrations of total SCFAs, ammonia nitrogen, and gas production, along with the proportions of butyrate, isobutyrate, and isovalerate, were markedly higher in the MG and FG groups compared with the EG group. Principal coordinate analysis highlighted significant variations in microbial communities among the EG, MG, and FG groups during the fermentation process. The abundances of Stomatobaculum and Blautia across the three groups increased over fermentation time, whereas Bacteroides , Lawsonibacter , Sporobacter , Papillibacter , Butyricicoccus , and Succiniclasticum exhibited higher abundances in MG or FG groups than in the EG group after 24 hours of fermentation. Co-occurrence network analysis uncovered positive correlations between butyrate levels and butyrate-producing bacteria ( Stomatobaculum , Butyrivibrio ) in the MG and FG groups. Additionally, there were positive correlations between proteolytic bacteria ( Clostridium and Roseburia ) and branched-chain volatile fatty acids in the FG group. These findings shed light on the fermentation patterns and microbial dynamics within the rumen of sika deer during different growth periods of velvet antler.

IMPORTANCE

Velvet antlers are distinctive and rapidly growing organs that hold significant value in traditional medicine. Through in vitro analysis, our study characterized the dynamics of microbiota and metabolites within the rumen liquid fermentation of sika deer throughout the different antler growth phase. We identified distinct microbial communities at various fermentation time points and observed shifts in fermentation patterns that paralleled antler development. These findings suggest a potentially pivotal role for these microbial dynamics in facilitating the growth process of velvet antlers.