Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis

• DOI: 10.1128/spectrum.03288-22
• Publication Date: 2023-01-11T14:03:57Z
• Authors: Ruyi Fan; Yi Zhou; Xu Chen; Xianmei Zhong; Fanzhen He; Wenzao Peng; Lu Li; Xiaoqian Wang; Yan Xu
• Keywords: 0303 health sciences; DNA methylation; apoptosis; Apoptosis; X-Ray Microtomography; DNA Methylation; Microbiology; QR1-502; Rats; 03 medical and health sciences; Humans; Animals; Tumor Suppressor Protein p53; Periodontitis; periodontitis; Porphyromonas gingivalis; outer membrane vesicles; Research Article

The outer membrane vesicles (OMVs) produced by Porphyromonas gingivalis contain a variety of bioactive molecules that may be involved in the progression periodontitis. However, participation P. OMVs development periodontitis has not been elucidated. Here, we isolated and confirmed their both vivo vitro. Microcomputed tomography (micro-CT) histological analysis showed under stimulation with OMVs, alveolar bone rats was significantly resorbed vivo. We found were taken up human periodontal ligament cells ([hPDLCs]) vitro, which subsequently resulted apoptosis inflammatory cytokine release, accomplished microRNA-size small RNA (msRNA) sRNA45033 OMVs. Through bioinformatics screening target genes, chromobox 5 (CBX5) identified as downstream screened-out sRNA45033. Using dual-luciferase reporter assay, overexpression, knockdown methods, to CBX5 regulate hPDLC apoptosis. In addition, CUT&Tag (cleavage targets tagmentation) mechanism regulates through methylation p53 DNA. Collectively, these findings indicate role is immunologically relevant related bacterial virulence during IMPORTANCE bacterium often associated This study demonstrates (i) CBX5, (ii) DNA its expression, apoptosis, (iii) novel interaction between hosts pathogens mediated occurrence