Detection and Quantification of Klebsiella pneumoniae in Fecal Samples Using Digital Droplet PCR in Comparison with Real-Time PCR

• DOI: 10.1128/spectrum.04249-22
• Publication Date: 2023-06-12T14:03:16Z
• Authors: Junxia Feng; Xiaohu Cui; Bing Du; Hanqing Zhao; Yanling Feng; Jinghua Cui; Chao Yan; Lin Gan; Zheng Fan; Tongtong Fu; Ziying Xu; Zihui Yu; Rui Zhang; Shuheng Du; Ziyan Tian; Qun Zhang; Guanhua Xue; Jing Yuan
• Keywords: Klebsiella pneumoniae; Feces; Humans; Reproducibility of Results; real-time PCR; Real-Time Polymerase Chain Reaction; Microbiology; Sensitivity and Specificity; quantification; QR1-502; 3. Good health; droplet digital PCR; Research Article

Klebsiella pneumoniae can cause a range of illnesses and has a high colonization rate in the human gut, making it crucial to develop an efficient method for detecting K. pneumoniae in fecal samples.