Escherichia coli ST2797 Is Abundant in Wastewater and Might Be a Novel Emerging Extended-Spectrum Beta-Lactamase E. coli

• DOI: 10.1128/spectrum.04486-22
• Publication Date: 2023-06-01T14:02:29Z
• Authors: Erik Paulshus; Patricia Colque; Inger Kühn; Tamanna Tauhid; Yue O. O. Hu; Yingshun Zhou; Kaisa Thorell; Roland Möllby; Henning Sørum; Åsa Sjöling; Enrique Joffré
• Keywords: Multilocus sequence typing; clone (Java method)

The increasing prevalence of antibiotic-resistant bacteria is an emerging threat to global health. analysis enterobacteria in wastewater can indicate the and spread certain clonal groups multiresistant bacteria. In a previous study Escherichia coli that were isolated from pump station Norway over 15 months, we found recurring E. clone was resistant trimethoprim, ampicillin, tetracycline 201 3,123 analyzed isolates (6.1%). 11 representative subjected whole-genome sequencing belong MLST ST2797 with plasmids carrying resistance genes, including blaTEM-1B, sul2, dfrA7, tetB. A phenotypic comparison uropathogenic ST131 ST648 repeatedly identified same samples revealed exhibited comparable capacity for temporal survival wastewater, greater biofilm formation, similar potential colonization mammalian epithelial cells. has been humans carry extended spectrum β-lactamase (ESBL) genes other studies, suggesting this type ESBL coli. Collectively, these findings show more ubiquitous studied than infamous may have abilities survive environment cause infections humans. IMPORTANCE incidence drug-resistant together levels infections. COVID-19 pandemic shed new light on importance monitoring threats finding early warning systems. Therefore, mitigate antimicrobial burden, identification hot spots, such as treatment plants, are required combat occurrence Here, applied PhenePlate system screening method genomic surveillance discovered dominant persistent multidrug pattern equivalent characteristics those major lineages, namely, ST648, which frequently genes. This highlights continuous report One Health settings.