Probiotic Lactobacillus Species and Their Biosurfactants Eliminate Acinetobacter baumannii Biofilm in Various Manners

• DOI: 10.1128/spectrum.04614-22
• Publication Date: 2023-03-15T14:03:17Z
• Authors: Mona Mohamed Al-S...; Jingdan Wang; Sirui Zhang; Pu Li; Woodvine Otieno O...; Yanjiong Chen; Bei Han; E. Yang; Meng Xun; Lei Han; Shaoshan Han
• Keywords: Acinetobacter baumannii; Lactobacillus rhamnosus

Acinetobacter baumannii is a critical biofilm-forming pathogen that has presented great challenges in the clinic due to multidrug resistance. Thus, new methods of intervention are needed control biofilm-associated infections. In this study, among three tested Lactobacillus species, rhamnosus showed significant antimaturation and antiadherence effects against A. biofilm. Lactic acid (LA) acetic (AA) were most effective antibiofilm biosurfactants (BSs) produced by L. rhamnosus. This phenomenon LA AA was strong bactericidal effect, which worked from very early time points, as determined colony enumeration confocal laser scanning microscope. The cell destruction appeared both envelope cytoplasm. A discontinuous envelope, leakage contents, increased extracellular activity ATPase demonstrated disruption membrane AA. These also occurrence protein lysis. addition, bacterial DNA interacted with damaged AA, resulting significantly reduced expression biofilm repair genes. results highlight possibility importance using probiotics clinical prevention. Probiotics can be utilized novel biocides block decrease formation microbial contamination medical equipment during treatment IMPORTANCE virulence factor causes colonization invasive illnesses. Rising resistance synthetic antimicrobials prompted researchers look at natural alternatives, such their derivatives. its BSs (LA AA) remarkable antimicrobial characteristics, inhibitory effect on baumannii. achieved several mechanisms, including membrane, lysis, biofilm-related genes, DNA. provide support for derivatives prevention therapy