Metabolite Alterations and Interactions with Microbiota in Helicobacter pylori-Associated Gastric Lesions

• DOI: 10.1128/spectrum.05347-22
• Publication Date: 2023-06-26T14:01:01Z
• Authors: Lei Peng; Yang Guo; Markus Gerhard; Juan-Juan Gao; Zong-Chao Liu; Raquel Mejías-Luque; Lian Zhang; Michael Vieth; Jun-Ling Ma; Wei-Dong Liu; Zhe-Xuan Li; Tong Zhou; Wen-Qing Li; Wei-Cheng You; Yang Zhang; Kai-Feng Pan
• Keywords: Helicobacter; Fusobacterium

Metabolites and their interactions with microbiota may be involved in Helicobacter pylori-associated gastric lesion development. This study aimed to explore metabolite alterations upon H. pylori eradication possible roles of microbiota-metabolite progression precancerous lesions. Targeted metabolomics assays 16S rRNA gene sequencing were conducted investigate metabolic microbial paired biopsy specimens 58 subjects successful 57 failed anti-H. treatment. Integrative analyses performed by combining the microbiome profiles from same intervention participants. A total 81 metabolites significantly altered after compared treatment, including acylcarnitines, ceramides, triacylglycerol, cholesterol esters, fatty acid, sphingolipids, glycerophospholipids, glycosylceramides, P values <0.05 for all. The differential showed significant correlations baseline specimens, such as negative between glycosylceramide, triacylglycerol (P < 0.05 all), which eradication. characteristic glycosylceramides Fusobacterium, Streptococcus, Gemella pylori-positive further noticed active gastritis intestinal metaplasia all). panel metabolites, genera, help discriminate high-risk who progressed mild advanced lesions short-term long-term follow-up periods areas under curve (AUC) 0.914 0.801, respectively. Therefore, our findings provide new insights into progression. IMPORTANCE In this study, a was established interactions, follow-up.