Increased apoptotic peripheral blood neutrophils in systemic lupus erythematosus: relations with disease activity, antibodies to double stranded DNA, and neutropenia

• DOI: 10.1136/ard.58.5.309
• Publication Date: 2008-12-06T00:32:33Z
• Authors: P A Courtney; A D Crockard; K Williamson; A E Irvine; R J Kennedy; A L Bell
• Keywords: Leukopenia

<h3>OBJECTIVE</h3> To quantify the percentage of apoptotic peripheral blood neutrophils in systemic lupus erythematosus (SLE) and to determine relations with disease activity neutropenia. <h3>METHODS</h3> Neutrophil apoptosis SLE patients (n =50) was assessed by flow cytometry using annexin V binding fluorescent labelled anti-fas. Rheumatoid arthritis (RA, n =20) inflammatory bowel (IBD, were studied as controls. <h3>RESULTS</h3> The neutrophils, determined binding, increased (median = 3.25%) compared normal healthy donors =20, median 1.20%) controls (RA: 1.15%) (IBD: 1.15%). neutrophil correlated positively measured SLAM score. antibodies dsDNA (&gt;10 mg/ml) had neutrophils. Eight 14 neutropenic Increased fas expression observed SLE, RA, IBD. <h3>CONCLUSION</h3> is non-specifically disease. circulating correlate (SLAM) may contribute autoantigen excess including dsDNA.