Interleukin 7 stimulates tumour necrosis factor α and Th1 cytokine production in joints of patients with rheumatoid arthritis

• DOI: 10.1136/ard.62.2.113
• Publication Date: 2003-01-14T01:04:25Z
• Authors: J A G van Roon; K A F M Glaudemans; J W J Bijlsma; F P J G Lafeber
• Keywords: Proinflammatory cytokine

<b>Background:</b> A large number of activated T cells are found in the joints patients with rheumatoid arthritis (RA). Interleukin 7 (IL7), a cell growth factor and regulator Th1 Th2 cytokine production, is produced by synoviocytes from RA. <b>Objective:</b> To investigate effect on proinflammatory production synovial fluid mononuclear (SFMC) mechanism which IL7 influences CD4+ activity <b>Methods:</b> In cross sectional group RA, levels were compared those healthy controls related to disease activity. The was tested RA SFMC SF presence (MC). Production tumour necrosis α (TNFα), IL1β, interferon γ (IFNγ), IL4 measured enzyme linked immunosorbent assay (ELISA) single FACS analysis. Expression receptor chain (essential for signalling) assessed. Direct effects isolated (SF) studied By neutralisation IL12 MC cultures, indirect through accessory studied. <b>Results:</b> serum higher than correlated positively C reactive protein levels. stimulated TNFα very potently IFNγ cells. These probably mediated chain, abundantly expressed Besides direct stimulation IL7, its action partly dependent IL12, indicating that also stimulates function, leading activation. <b>Conclusion:</b> intra-articular correlation measures indicates might substantially contribute perpetuation responses