Cytomegalovirus DNA detection on Guthrie cards in patients with neonatal cholestasis

• DOI: 10.1136/fn.80.2.f130
• Publication Date: 2009-04-08T15:39:47Z
• Authors: B. Fischler; P. Rodensjo; A. Nemeth; M. Forsgren; I. Lewensohn-Fuchs
• Keywords: Cytomegalovirus

<h3>AIM</h3> To time the onset of cytomegalovirus (CMV) infection in patients (n=39) with CMV associated neonatal cholestasis by analysing DNA on Guthrie cards sampled at 3 days age. <h3>METHODS</h3> was diagnosed serology/urine isolation or detection (polymerase chain reaction) liver biopsy specimens. In order to dry blood filter paper discs were punched out from stored cards. After phenol–chloroform extraction detected nested polymerase reaction. <h3>RESULTS</h3> All control children (n=8) congenital tested positive; none negative controls (n=4) did so. Two 39 cholestatic infants their mothers had serological signs compatible during second half pregnancy. other negative. <h3>CONCLUSIONS</h3> not most using cards, suggesting that developed soon after birth.