Anti-monocyte chemoattractant protein 1 gene therapy attenuates experimental chronic pancreatitis induced by dibutyltin dichloride in rats

• DOI: 10.1136/gut.2004.049403
• Publication Date: 2005-11-11T19:03:13Z
• Authors: H F Zhao
• Keywords: Monocyte

Monocyte chemoattractant protein 1 (MCP-1) is a member of the C-C chemokine family and exerts strong activity in monocytes, macrophages, lymphocytes. Rat pancreatic fibrosis induced by dibutyltin dichloride (DBTC) considered to be an appropriate chronic pancreatitis model histologically enzymatically, as has demonstrated previous study.We examined effect human dominant negative inhibitor MCP-1 (mutant MCP-1) on progression DBTC rat model.We used experimental rats. Mutant or empty plasmid at dose 50 microg/body weight was administrated into thigh muscles days 4, 11, 18 after administration DBTC. On 14 28, we evaluated mutant morphologically biochemically.The treated group inhibited early inflammation later histologically, showed decrease serum concentration, intrapancreatic hydroxyproline, alpha-smooth muscle actin, increase amylase content compared with group. The also mRNA expression cytokines chemokines.: Our findings suggest that monocyte/macrophage recruitment systemic signal pathway contribute pancreatitis, blockade may suppress development fibrosis.