Intratumoral TIGIT+ CD8+ T-cell infiltration determines poor prognosis and immune evasion in patients with muscle-invasive bladder cancer

• DOI: 10.1136/jitc-2020-000978
• Publication Date: 2020-08-17T07:00:27Z
• Authors: Zhaopei Liu; Quan Zhou; Zewei Wang; Hongyu Zhang; Han Zeng; Qiuren Huang; Yifan Chen; Wenbin Jiang; Zhiyuan Lin; Yang Qu; Ying Xiong; Qi Bai; Yu Xia; Yiwei Wang; Li Liu; Yu Zhu; Le Xu; Bo Dai; Jianming Guo; Jiajun Wang; Yuan Chang; Weijuan Zhang
• Keywords: TIGIT

T-cell immunoglobulin and ITIM domain (TIGIT) is identified as a novel checkpoint receptor that can facilitate immune escape via mediating exhaustion in tumors. However, the clinical significance contexture correlation of intratumoral TIGIT+ CD8+ T-cells remain to be further explored muscle-invasive bladder cancer (MIBC).259 patients with MIBC from two centers (Zhongshan Hospital, n=141; Shanghai Cancer Center, n=118) were analyzed evaluate prognostic value association through immunohistochemistry. Fresh tumor tissue samples 26 examined discover phenotype this CD8 subpopulation by flow cytometry.High infiltration predicted poor overall survival (OS) recurrence-free (RFS) MIBC. For stage II low cells, adjuvant chemotherapy (ACT) could significantly prolong their OS RFS. Intratumoral abundance was correlated impaired cytotoxicity exhibited production immunosuppressive cytokine IL-10. Further analysis tumor-infiltrating cell landscape revealed associated suppressive contexture, including Th2 regulatory T-cells, mast cells neutrophils.Intratumoral serve an independent prognosticator for outcome predictive biomarker inferior ACT responsiveness. dampened antitumor immunity abundance, highlighting tumor-promoting role T-cells.