Design, synthesis and immunological evaluation of self-assembled antigenic peptides from dual-antigen targets: a broad-spectrum candidate for an effective antibreast cancer therapy

• DOI: 10.1136/jitc-2021-002523
• Publication Date: 2021-06-03T17:14:04Z
• Authors: Wei Shi; Qianqian Qiu; Ziying Feng; Zhenzhen Tong; Weiwei Guo; Feng Zou; Na Yue; Wenlong Huang; Hai Qian
• Keywords: Cancer Immunotherapy

Background Considering the narrow immune response spectrum of a single epitope, and nanoparticles (NPs) as novel adjuvant can achieve efficient delivery antigenic peptides safely, nano-system (denoted DSPE-PEG-Man@EM-NPs) based on cathepsin B-responsive was designed synthesized. Methods Highly affinitive were delivered by self-assembled NPs, targeted erythrocyte membranes acted peptide carrier to improve presentation strengthen cytotoxic T-cells reaction. Cathepsin B coupling could release rapidly in dendritic cells. Results Evaluations showed that DSPE-PEG-Man@EM-NPs had obvious inhibitory effects towards both MCF-7 MDA-MB-231 human breast cancer cell lines. Conclusion Overall, this strategy provides for boosting T lymphocytes response, thereby expanding adaptation range tumor improving therapeutic effect immunotherapy with nanomedicine.