Enhanced thrombopoiesis supplies PD-L1 to circulating immune cells via the generation of PD-L1-expressing platelets in patients with lung cancer

• DOI: 10.1136/jitc-2024-010193
• Publication Date: 2025-02-26T17:53:41Z
• Authors: Sung-Woo Lee; Saei Jeong; Young Ju Kim; Jeong Eun Noh; Kyung Na Rho; Hee-Ok Kim; Hyun-Ju Cho; Deok Hwan Yang; Eu Chang Hwang; Woo Kyun Bae; Sook Jung Yun; Ju Sik Yun; Cheol-Kyu Park; In-Jae Oh; Jae-Ho Cho
• Keywords: Thrombopoiesis

Background The increased expression of programmed cell death ligand 1 (PD-L1) on a subset immune cells in the peripheral blood has been frequently observed patients with cancer, suggesting relationship PD-L1 tumor tissues. In this study, we investigated mechanisms underlying various types cancer. Methods populations was analyzed mononuclear 112 non-small lung cancer (NSCLC) using flow cytometry. A mouse model X-ray-induced acute thrombocytopenia used to investigate between thrombopoiesis and PD-L1-expressing platelet generation. clinical significance platelets cohort stage IV NSCLC who received combination anti-programmed (PD-1) therapy chemotherapy. Results All populations, including monocytes, T cells, B NK showed higher than healthy controls. However, frequency not attributed themselves. Instead, it entirely dependent direct interaction platelets. Notably, platelet-dependent acquisition circulating other mechanistically associated surge thrombopoiesis, resulting production reticulated Clinically, enhanced concurrently high exhibited better response anti-PD-1 therapy. Conclusions These findings highlight role tumor-associated generating that may serve as resource for PD-L1-positive circulation act predictive biomarker anti-PD-1/PD-L1