Chromosome 3p allele loss in early invasive breast cancer: detailed mapping and association with clinicopathological features

• DOI: 10.1136/mp.54.5.300
• Publication Date: 2002-07-27T10:30:44Z
• Authors: A Martinez
• Keywords: Paraffin Embedding; Carcinoma, Ductal, Breast; Loss of Heterozygosity; Breast Neoplasms; Physical Chromosome Mapping; Polymerase Chain Reaction; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; Receptors, Estrogen; Case-Control Studies; Biomarkers, Tumor; Humans; Female; Chromosomes, Human, Pair 3; Receptors, Progesterone; Microsatellite Repeats

<i>Aims</i>—Chromosome 3p allele loss is a frequent event in many common sporadic cancers including lung, breast, kidney, ovarian, and head neck cancer. To analyse the extent frequency of allelic losses T1N0 T1N1 invasive breast cancer, 19 microsatellite markers spread along were analysed 40 such carcinomas with known clinicopathological parameters. <i>Methods</i>—Loss heterozygosity analysis was carried out using that non-randomly distributed chosen to represent regions show hemizygous and/or homozygous lung cancer (lung tumour suppressor gene region 1 ( LCTSGR1) at 3p21.3 LCTSGR2 3p12), demonstrating suppression tumorigenicity ovarian <i>Results</i>—Allelic seen one or more loci 22 these clinically early stage tumours, but none had complete loss. Several non-overlapping deletions defined, namely: (1) 18 tumours showed 3p21–22, physical distance 12 Mb; (2) 11 3p12 within Mb, this contained LCTSGR2; (3) six 3p25–24, von Hippel-Lindau (VHL) locus; (4) five 3p14.2, fragile histidine triad (FHIT) locus. <i>Conclusions</i>—This largest study date defining range results indicate 3p21–22 containing LCTSGR1 are most sites carcinomas. This suggests genes located may play important roles development There an association between increasing grade progesterone (p = 0.0098) oestrogen 0.0472) receptor expression, indicating link regulation differentiation.