EP122 Involvement of the spinal γ-aminobutyric acid receptor in the analgesic effects of intrathecally injected hypertonic saline in spinal nerve-ligated rats

• DOI: 10.1136/rapm-2023-esra.184
• Publication Date: 2023-10-10T10:32:09Z
• Authors: Yujin Kim; Myong-Hwan Karm; Hyun-Jung Kwon; Euiyong Shin; Seung-Hwa Ryoo; Sooyoung Jeon; Seong-Soo Choi
• Keywords: Hypertonic saline; Allodynia; Hot plate test

<h3>Background and Aims</h3> Hypertonic saline is used for treating chronic pain, but clinical studies with optimal therapy protocols are lacking. This study aimed to determine the concentration at which effect reaches its peak antinociceptive mechanism of saline. <h3>Methods</h3> A spinal nerve ligation (SNL, left L5, L6) model was induce neuropathic pain in rats weighing 250–300 g. One week after implantation intrathecal catheter, different concentrations NaCl were injected intrathecally into rats. Behavioral tests (von Frey filaments, hot-plate, cold-plate tests) derive results baseline, 30 minutes, 2 hour, 1 day, week. After same preparation, randomly divided four groups 10: control group, hypertonic bicuculline phaclofen group. then performed weeks 3 each drug administration, followed administration 5% NaCl. reviewed apporoved by Institutional Animal Care Use Committee Asan Institute Life Sciences. <h3>Results</h3> Using more than induced mechanical allodynia thermal hyperalgesia has a significant therapeutic effect. Moreover, showed partial time- dose-dependent on cold hyperalgesia. Pretreatment γ-Aminobutyric Acid (GABA) receptor antagonist inhibited SNL <h3>Conclusions</h3> Intrathecally effective greater effects may be associated GABAA GABAB receptors.