Effect of inhaled glucocorticoids on IL-1 beta and IL-1 receptor antagonist (IL-1 ra) expression in asthmatic bronchial epithelium

• DOI: 10.1136/thx.52.5.407
• Publication Date: 2010-01-11T19:40:22Z
• Authors: A. R. Sousa; C. J. Trigg; S. J. Lane; R. Hawksworth; J. A. Nakhosteen; R. N. Poston; T. H. Lee
• Keywords: BETA (programming language)

BACKGROUND: Accumulating evidence suggests that the cytokine network is central to immunopathology of bronchial asthma and existence naturally occurring antagonists has added this complexity. Upregulation both interleukin 1 beta (IL-1 beta) its receptor antagonist, antagonist (IL-1ra), previously been observed on asthmatic epithelium compared with normal airways. METHODS: The effect inhaled beclomethasone dipropionate (BDP) epithelial expression IL-1 IL-1ra was studied. Frozen biopsy specimens from nine subjects receiving 1000 micrograms BDP daily for eight weeks six matching placebo were stained anti-IL-1 anti-IL-1ra antibodies. Hue-saturation-intensity (HSI) colour image analysis used quantify brown immunoperoxidase reaction present epithelium. RESULTS: There a significant twofold decrease in after treatment but no change seen (P = 0.175). CONCLUSION: selective inhibition beta, without IL-1ra, provides novel mechanism anti-inflammatory action glucocorticosteroids.